Transient detection of Chlamydial-specific Th1 memory cells in the peripheral circulation of women with history of Chlamydia trachomatis genital tract infection.
Vicetti Miguel RD1, Reighard SD, Chavez JM, Rabe LK, Maryak SA, Wiesenfeld HC, Cherpes TL.
1Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224, USA.
Abstract
PROBLEM:
Development
of safe and effective Chlamydia trachomatis vaccines requires better
understanding of the host immune responses elicited by natural
infection.
METHOD OF STUDY:
Peripheral
blood mononuclear cells isolated from women with or without history of
genital tract chlamydial infection were stimulated with inactivated
C. trachomatis elementary bodies (EB) in ELISPOT assays that enumerated
frequencies of cells producing interferon (IFN)-γ or interleukin
(IL)-17.
RESULTS:
IFN-γ-positive
cells were highest among women sampled 30-60 days after diagnosis of
C. trachomatis infection and treatment initiation, while the numbers of
IFN-γ-positive cells were equally low among uninfected women and women
sampled <30 or >60 days after diagnosis of infection. Conversely,
IL-17-positive cell numbers were uniformly low among all participants.
CONCLUSION:
Dramatically
reduced numbers of Chlamydia-specific Th1 memory cells in the
peripheral circulation of study participants sampled more than 2 months
after diagnosis, and initiation of treatment provides new insight into
the results from C. trachomatis vaccine trials, in which immunization
with EB provided only short-lived protection. Our results also suggest
that an effective vaccine against this weakly antigenic intracellular
pathogen will need to generate immunological memory more durable than
that elicited by natural infection.
© 2012 John Wiley & Sons A/S.
Am J Reprod Immunol. 2012 Dec;68(6):499-506. doi: 10.1111/aji.12008. Epub 2012 Aug 31.
http://onlinelibrary.wiley.com/doi/10.1111/aji.12008/abstract;jsessionid=B95D7E5E29926F60FB0D65C3E2DB60D8.f02t01
Md. Rodolfo Vicetti. Ex-miembro del GII.
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