Dieta y Sindrome Premenstrual

Dietary vitamin D intake, 25-hydroxyvitamin D3 levels and premenstrual syndrome in a college-aged population.

Bertone-Johnson ER¹, Chocano-Bedoya PO, Zagarins SE, Micka AE, Ronnenberg AG.

¹Division of Biostatistics and Epidemiology, Department of Public Health, University of Massachusetts, Amherst, MA 01003-9304, USA. ebertone@schoolph.umass.edu

 

Abstract

High dietary intake of vitamin D may reduce the risk of premenstrual syndrome (PMS), perhaps by affecting calcium levels, cyclic sex steroid hormone fluctuations, and/or neurotransmitter function. Only a small number of previous studies have evaluated this relationship and none have focused on young women. We assessed this relationship in a cross-sectional analysis within the UMass Vitamin D Status Study. Between 2006 and 2008, 186 women aged 18-30 (mean age=21.6 years) completed a validated food frequency questionnaire, additional questionnaires to assess menstrual symptoms and other health and lifestyle factors, and provided a fasting blood sample collected during the late luteal phase of their menstrual cycle. Among all study participants, results suggested the possibility of an inverse association between intake of vitamin D from food sources and overall menstrual symptom severity, though were not statistically significant; mean intakes in women reporting menstrual symptom severity of none/minimal, mild, and moderate/severe were 253, 214, and 194 IU/day, respectively (P=0.18). From among all study participants, 44 women meeting standard criteria for PMS and 46 women meeting control criteria were included in additional case-control analyses. In these women, after adjustment for age, body mass index, smoking status and total calcium intake, higher intake of vitamin D from foods was associated with a significant lower prevalence of PMS. Women reporting vitamin D intake from food sources of >or=100 IU/day had a prevalence odds ratio of 0.31 compared to those reporting<100 IU/day (95% confidence interval=0.10-0.98). Late luteal phase 25-hydroxyvitamin D3 levels were not associated with prevalent PMS. Results from this pilot study suggest that a relationship between vitamin D and PMS is possible, though larger studies are needed to further evaluate this relationship and to investigate whether 25-hydroxyvitamin D3 levels in the follicular or early luteal phases of the menstrual cycle may be related to PMS risk.

Copyright (c) 2010 Elsevier Ltd. All rights reserved.

J Steroid Biochem Mol Biol. 2010 Jul;121(1-2):434-7. doi: 10.1016/j.jsbmb.2010.03.076. Epub 2010 Apr 14.

http://www.sciencedirect.com/science/article/pii/S0960076010001755

Md. PhD. Patricia Chocano. Ex miembro del GII.   

Cancer y Citocinas

Actin cytoskeleton participation in the onset of IL-1beta induction of an invasive mesenchymal-like phenotype in epithelial MCF-7 cells.

 

Franco-Barraza J1, Valdivia-Silva JE, Zamudio-Meza H, Castillo A, García-Zepeda EA, Benítez-Bribiesca L, Meza I.

¹Departamento de Biomedicina Molecular, CINVESTAV-IPN, Apartado, México, D.F., México.

Abstract

BACKGROUND:

Interleukin 1 beta (IL-1beta) and other inflammatory cytokines are reported to induce phenotypic changes in epithelial breast cancer tumor cells related to increased invasiveness. Mechanisms involved in the process are not well understood.

METHODS:

The noninvasive breast cancer epithelial cell line MCF-7 was used to investigate the IL-1beta-induced phenotype. Live cells expressing EGFP-actin were monitored for cell morphology changes and actin cytoskeleton dynamics by time-lapse video microscopy in the presence of IL-1beta and specific inhibitors of actin signaling pathways. Chemotaxis, invasion of Matrigel, MMP activity and expression of S100A4 in cells treated with IL-1beta were assessed by migration assays, zymograms and immunoblots.

RESULTS:

Exposure to IL-1beta specifically induced a change in MCF-7 cells from a typical epithelial morphology into elongated cells, showing numerous dynamic actin-rich lamellae and peripheral ruffles characteristic of fibroblasts. These cells could scatter from compact cell colonies and respond to chemoattractants such as the homing-associated chemokine CXCL-12. Pharmacological blockage of actin signaling pathways and negative mutants of RhoGTPases revealed that actin reorganization and enhanced motility are regulated via PI3K/Rac 1 activation. IL-1beta-stimulated cells expressed the metastasis promoter S100A4, increased secretion of active MMP-9 and MMP-2 and invasion of extracellular matrix proteins.

CONCLUSIONS:

IL-1beta induces a PI3K/Rac 1-regulated reorganization of the actin cytoskeleton of MCF-7 cells that is required for cell scattering, elongation and migration. The enhanced motility is accompanied by expression of protein markers correlated with invasive behavior.
Copyright 2010 IMSS. Published by Elsevier Inc. All rights reserved.

Arch Med Res. 2010 Apr;41(3):170-81. doi: 10.1016/j.arcmed.2010.04.010.

http://www.sciencedirect.com/science/article/pii/S0188440910001013

Md PhD Julio Valdivia Silva. Fundador del GII

Influenza

Changes in the viral distribution pattern after the appearance of the novel influenza A H1N1 (pH1N1) virus in influenza-like illness patients in Peru.

 

Laguna-Torres VA1, Gómez J, Aguilar PV, Ampuero JS, Munayco C, Ocaña V, Pérez J, Gamero ME, Arrasco JC, Paz I, Chávez E, Cruz R, Chavez J, Mendocilla S, Gomez E, Antigoni J, Gonzalez S, Tejada C, Chowell G, Kochel TJ; Peru Influenza working group.

 ¹Virology Department, United States Naval Medical Research Center Detachment, Lima, Perú. alberto.laguna@med.navy.mil

 

Abstract

BACKGROUND:

We describe the temporal variation in viral agents detected in influenza like illness (ILI) patients before and after the appearance of the ongoing pandemic influenza A (H1N1) (pH1N1) in Peru between 4-January and 13-July 2009.

METHODS:

At the health centers, one oropharyngeal swab was obtained for viral isolation. From epidemiological week (EW) 1 to 18, at the US Naval Medical Research Center Detachment (NMRCD) in Lima, the specimens were inoculated into four cell lines for virus isolation. In addition, from EW 19 to 28, the specimens were also analyzed by real time-polymerase-chain-reaction (rRT-PCR).

RESULTS:

We enrolled 2,872 patients: 1,422 cases before the appearance of the pH1N1 virus, and 1,450 during the pandemic. Non-pH1N1 influenza A virus was the predominant viral strain circulating in Peru through (EW) 18, representing 57.8% of the confirmed cases; however, this predominance shifted to pH1N1 (51.5%) from EW 19-28. During this study period, most of pH1N1 cases were diagnosed in the capital city (Lima) followed by other cities including Cusco and Trujillo. In contrast, novel influenza cases were essentially absent in the tropical rain forest (jungle) cities during our study period. The city of Iquitos (Jungle) had the highest number of influenza B cases and only one pH1N1 case.

CONCLUSIONS:

The viral distribution in Peru changed upon the introduction of the pH1N1 virus compared to previous months. Although influenza A viruses continue to be the predominant viral pathogen, the pH1N1 virus predominated over the other influenza A viruses.

PLoS One. 2010 Jul 27;5(7):e11719. doi: 10.1371/journal.pone.0011719.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0011719

Md. Irmia Paz. Tutora del GII

Ojé y Coagulación sanguínea

 Efecto in vitro del látex de Ficus insipida sobre la cascada de la coagulación sanguínea.

Fernando Concha-Benavente ¹

1Médico Cirujano miembro del Grupo de Investigación en Inmunología y Academia Peruana de Medicina Molecular. Facultad de Medicina de la Universidad Nacional de San Agustín. Arequipa, Perú.

SUMMARY
We need to search for new natural drugs yielding pharmacological active principles to be used as an alternative to conventional therapies. For this reason, we proposed to study Ficus insipida, an amazonian plant that its latex has been used for years as an Antihelmintic agent but its anticoagulant effect has been only superficially studied. Objective: To corroborate the in vitro anticoagulant effect and to determine upon which of the coagulation pathways acts the latex of Ficus insipida. Material and methods: The latex of Ficus insipida was obtained and prepared at different concentrations. Then, samples of peripheral blood from 5 donors were taken using sodium citrate to avoid blood clotting; the samples were mixed with the latex dilutions and centrifuged, after that, the plasma was extracted to form a plasma pool from each latex concentration. Finally, the Prothrombin Time (PT) and the Partial Thromboplastin Time (PTT) were determined, respectively. Results: The results obtained demonstrated that the latex of Ficus insipida delays the PT at concentrations equal or higher than 0.03125% (V/V), and both the PT and PTT at concentrations equal or higher than 0.15% (V/V). Conclusions: This data allow us to affirm that the latex of Ficus insipida has a dose-dependent in vitro anticoagulant effect upon the extrinsic coagulation pathway at concentrations equal or higher than

0.03125% (V/V). Additionally, at concentrations equal or higher than 0.15% (V/V) it has a potent anticoagulant effect over both coagulation pathways.(Rev Med Hered 2010;21:146-152).

KEY WORDS: Blood coagulation factors, prothrombin time, partial thromboplastin time, ficus, anticoagulant agents.

 

Rev Med Hered v.21 n.3 Lima jul. 2010

http://www.scielo.org.pe/scielo.php?script=sci_arttext&pid=S1018-130X2010000300006

Md.PhD. Fernando Concha-Benavente. Ex-miembro del GII